FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004998886

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004998886
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40900009
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA690020.16870900933053584No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG648970.1586723367224687No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG119300.014.766047545
GTTGTCG18150.013.38743926
GGCATAG132600.013.11477635
ATAGGGG136050.013.09680538
GAGCTTA149000.011.94357144
AGGGATC146200.011.8060912
GGGAGGT216000.011.7312113
TGGCATA153100.011.46160334
CATAGGG156950.011.32405237
GGAGGTC225700.010.9081984
GTCACTC223350.010.5890088
GCATAGG171550.010.36013736
ATTTCTA358750.010.337692
ACTCTCC228050.010.33091311
GGTCACT231750.010.1086317
AACTCTA80500.010.06119545
TCAGACG73950.09.9510242
CGTGGCG114600.09.9185911
CAGGGAT194500.09.9059781
GGGATCA187450.09.6042353
Produced by FastQC (version 0.11.5)