FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004998888

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004998888
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37575209
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1180370.31413531192867084No Hit
CTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAG642420.17096910891433764No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG402950.10723825914048808No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC237250.025.2185634
GACCGCG244050.024.4951745
CAGACCG248050.024.3382033
ACCGCGT245900.024.246846
TCAGACC272100.022.542622
TTCGGCG85900.022.47077244
TTCGACG22350.022.3482346
TCGGCGC89600.021.79393245
CCGCGTT285350.020.8537047
CGGTATA34800.020.36712520
CGCGTTC298050.019.9646978
TCGACGA25050.019.9379587
CTCAGAC332600.019.7246061
GTTCGAC26650.018.5734795
GCGTTCT337700.017.6738749
CCGTACG114050.017.223734
CGTACGC115150.017.0576085
CGACGAG29100.016.9307928
CTACACG67050.016.20586645
ATTCGGC120000.016.19783443
Produced by FastQC (version 0.11.5)