FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004998898

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004998898
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33949766
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG479160.14113793891834187No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA439820.12955023018420805No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG19400.017.8501836
CGGTATA28650.016.64809820
CCGGCTT171850.015.8590592
GCCGGCT153850.015.7759931
GTCCGTT28350.015.7103161
CCCGTAT25400.015.5866121
AACGCTA168600.015.4196223
TCGACGA23600.015.3405227
ACGCTAC170550.015.3106324
CGACGAG22800.015.188188
GACCGCG27750.014.9910855
TCACGCT179400.014.9658628
TTCACGC181100.014.8131797
CGCTACC176300.014.6705625
ACGCTCA185750.014.53898310
CTACACG60000.014.50705945
GCGGACT45700.014.4662342
GTTCGAC23000.014.3718885
AAAACGC186000.014.11671721
GAAAACG190400.013.80159520
Produced by FastQC (version 0.11.5)