FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004998900

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004998900
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46756136
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT648720.1387454258410062No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGCG149200.020.6748125
AGACCGC152250.020.275434
ACCGCGT156000.019.7736686
TTCGGCG71100.018.9450344
TTCGACG23750.018.554546
CAGACCG168350.018.5501063
CCGCGTT178900.017.2299827
GTCGGTT43050.017.02651
AGTTACC89200.016.9480434
TCGGCGC79350.016.86212545
CCTACTC92750.016.65339918
CGCGTTC186800.016.5615048
GTTCCTA96600.015.98962415
TCGACGA29950.015.839577
TCAGACC208450.015.3159092
GCACTAC67950.015.23070325
GTCCGTT36600.015.1124531
CGGTATA42250.015.08209520
AACGCTA92550.014.87439623
ACGCTAC94050.014.64802824
Produced by FastQC (version 0.11.5)