FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004998904

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004998904
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30551176
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAG506680.16584631635783842No Hit
CTGGGCTTGTGATTCACGTTGCAGATGTAGGTCTGGGTGCCCAAGCTGCTG383550.12554344880210178No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC46600.018.43134
CAGACCG52250.016.7825053
TCGGCGC64100.016.73387145
ACCGCGT52400.016.6915286
TTCGGCG66450.016.34488344
GTTATAA82600.016.27970540
CCGCGTT88150.015.9955981
TTCGACG7600.015.9756296
CGCGTGT9250.015.8025411
AGTTATA75150.015.38016439
TAGTTAT74900.015.31085938
ACGTTCG10700.014.9194356
TTCGCGC10050.014.765787
AACGCTA71950.014.71352523
GTCCGTT17350.014.6465131
ACGCTAC73500.014.45656924
CGTACGC77650.014.0146085
TTATAAC94250.013.814141
CCGTACG79250.013.6182244
CGCTACC69600.013.58119125
Produced by FastQC (version 0.11.5)