FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004998908

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004998908
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41682958
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1585260.3803137003856588No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC291850.027.8749644
GACCGCG295600.027.4147535
CAGACCG297600.027.291013
ACCGCGT301000.026.9287246
CCGCGTT316350.025.6160627
CGCGTTC330450.024.5502228
TCAGACC339250.024.5111642
GAACGAT340950.023.41209845
GCGTTCT356400.022.7689959
CTCAGAC385850.022.6499481
ATACGGA376200.021.313233
AATACGG376700.021.2878732
AGAACGA380250.021.21130444
TACGGAG380750.020.98343334
CAATACG392200.020.52574331
AAGAACG401750.020.0593643
ACGGAGA401050.020.02795835
CCCAATA413900.019.61683729
CGTTCTC428200.019.28737610
CCAATAC431550.018.90324230
Produced by FastQC (version 0.11.5)