FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004998934

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004998934
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39867583
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG29600.024.0040686
GTTCGAC34000.020.5035695
TCGACGA36400.020.0139647
CGGTATA43850.019.90446320
GACCGCG50600.018.3546525
CGACGAG38050.018.2005948
ACCGCGT54500.017.2864976
AGACCGC57600.016.5926954
CAGACCG63500.015.4405673
ACGAACG41450.015.16218631
CCGCGTT63600.014.7070547
CGTACGC41750.014.3275465
CCGTACG42650.014.2889634
ACGAGGA65250.013.8182610
CGCGTTC69350.013.617348
AACGCTA89100.013.57043623
AGTTCGA53700.013.5262914
CTCCGTT57100.013.4814681
CCCGTAT28150.013.4331581
ACGCTAC91550.013.42978824
Produced by FastQC (version 0.11.5)