FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004998935

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004998935
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39867583
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN798710.20034071290451697No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGGG92100.012.36065838
AGCTTAG92750.012.12444845
GGCATAG95050.011.59196435
CATAGGG107300.010.41878937
GCATAGG112250.09.93712636
GAGCTTA116500.09.84531244
GTCACTC145200.09.73267658
GGGAGGT170250.09.6685653
TCAGACG63600.09.2766282
TGGCATA126200.09.06806634
GGAGGTC174000.08.9684284
CGTGGCG87250.08.8785321
GGTCACT158100.08.8534277
AGGTCAC166550.08.8233266
AACTCTA90650.08.55196745
ACTCTCC173550.08.40175311
GAGGTCA190200.08.3936865
TAGGGGA172850.08.34577639
CGACCCG92300.08.2897426
ATATAGC33050.08.1028796
Produced by FastQC (version 0.11.5)