FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004998936

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004998936
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20998743
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1158440.5516711166949374No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG364640.17364848934052862No Hit
CTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAG291260.13870354049287617No Hit
CTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACACG286430.13640340281320648No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACG246960.11760703962137162No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA243550.11598313289514521No Hit
CCCAGCTTCATCTTCAACGTTGTGGAAAGGGACTGTACATCATGGGGCAGA212940.10140606987761125No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG34050.032.1698726
TCGACGA37300.029.4270977
GTTCGAC36650.029.396875
CGACGAG38400.027.76418
CGGTATA50950.027.56205620
AGACCGC249150.025.5936814
GACCGCG250550.025.3877685
CAGACCG253650.025.3707243
AGTTCGA43150.025.1250444
ACCGCGT256100.024.8199026
TCAGACC269650.024.0322112
CTCAGAC285000.023.8540291
CCGCGTT281550.022.5843057
CGCGTTC286150.022.1505078
ACGAGGA59900.021.96673410
CAGTTCG51650.021.7310183
GACGAGG60950.021.5883089
CGAGGAT57400.021.04257211
GCGTTCT302450.020.941879
ATGACGG64150.020.7217916
Produced by FastQC (version 0.11.5)