FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004998942

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004998942
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50750146
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT608210.11984399020251095No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC146550.022.0612724
ACCGCGT149350.021.6311956
GACCGCG152150.021.2183135
CAGACCG163800.020.247163
CCGCGTT164400.019.5960987
TTCGACG21850.019.1510626
CGCGTTC170350.018.7925078
TCAGACC204950.016.4783172
CCGTACG76800.015.3800474
CGGTATA39500.014.98153520
CGTACGC77500.014.9498365
GCGTTCT219050.014.7374969
TCGACGA29200.014.4844947
GCACTAC72150.014.37868625
TTCGGCG78850.014.15152344
GTCGGTT45650.014.0615131
TCGGCGC80250.014.04468545
GAACGAT227100.013.88833745
CTCAGAC276300.013.8660071
TACGCCA84100.013.8032717
Produced by FastQC (version 0.11.5)