FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004998947

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004998947
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32462212
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN655670.20197945845464874No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG21950.09.832314526
TCTATAG157950.09.69434453
ATAGTGT174150.09.2136986
CTATAGT164600.09.2068494
TAGTGTG175000.08.9887997
TATAGTG175450.08.9195545
ATAGGGG58350.08.71418538
CGACCCG60650.08.5713476
TACTAGA178250.08.54656215
TTACTAG178700.08.52552814
CGTGGCG62900.08.4489991
AAGCCGG122350.08.36219745
TTCTATA190300.08.3424752
AACTCTA76750.08.25080745
ATATAGC33200.08.2019156
GTTACTA185500.08.17678513
CACCGAA186750.08.13331945
TGTCGTT26050.08.025528
TAGCGTT127100.07.925927624
GCACCGA190500.07.913575644
Produced by FastQC (version 0.11.5)