FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004998952

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004998952
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14083320
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA533220.3786181099343052No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG410310.2913446545274836No Hit
CTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAG371240.26360261642851257No Hit
CTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACACG185540.13174450342674882No Hit
CTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACG184660.1311196507641664No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT174040.12357881522254695No Hit
CCCAGCTTCATCTTCAACGTTGTGGAAAGGGACTGTACATCATGGGGCAGA155210.11020838836297124No Hit
CCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAA146750.10420128208405405No Hit
CCCGTCCCTGCTGACACCAACCATTCTTACCGGTAGCCTGCCAACACACAA141520.10048766910075181No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG16900.027.2937646
GTTCGAC18800.024.308935
TACGCGA651.5274363E-524.2264620
TCGACGA19500.024.2262027
CGGTATA25000.023.30585520
AGTTCGA23150.020.1310064
CCGGCTT151400.019.7484872
CCCCCGT13500.019.3558711
GCCGGCT133550.018.3853171
CAGTTCG28450.017.8848253
CGAGGAT30700.017.87936611
CGGCTTC171600.017.6729413
TTCACGC164900.017.4618387
CTACACG70300.017.45592745
TCACGCT165850.017.3211258
CGACGAG28250.017.2002988
CCCGGCT32400.017.1727751
GTATAGA37650.016.8498322
ATGACGG34450.016.4556516
ACGCTCA177250.016.24517410
Produced by FastQC (version 0.11.5)