FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004998962

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004998962
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37220679
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT2652600.7126683529873273No Hit
CCCAAATCTTTAAACCGATGAGCAACCTCACTCTTGTGTGCATCTCGACGA886830.23826271412189984No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACG558780.15012622418844104No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA518250.13923711601284866No Hit
GGACACTGCTGAAGATACTGAGCAAAGGCAATCAACACCAAGGCTTTGAAA379970.10208572498099779No Hit
CTTACTTTCAACAAAATCAGCAGCTAATGAAGGCAAGTCAGCAGGCATCTC379100.1018519839468807No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT482700.027.07619745
AGACCGC490350.027.035834
CAGACCG499100.026.6119923
GACCGCG499400.026.563735
ACCGCGT506050.026.2322926
TCAGACC517000.025.8169562
CCGCGTT544000.024.4105727
CGCGTTC556500.023.850148
CTCAGAC581550.023.7469061
AGAACGA556850.023.4873744
GCGTTCT575350.023.103939
CGTTCTC600000.022.25970610
TTCGACG11250.021.192586
AAGAACG615900.021.1540643
ATACGGA634400.020.6387733
TACGGAG638550.020.5002834
AATACGG638900.020.49051732
CAATACG641350.020.41764531
ACGGAGA653350.020.05249835
GTTCTCT679950.019.70521211
Produced by FastQC (version 0.11.5)