FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004998978

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004998978
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44598217
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG455720.1021834572444903No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG31200.024.3602336
TCGACGA38550.020.7075257
GTTCGAC37350.020.6501085
CGGTATA55900.020.32489620
CGACGAG40200.018.6827478
AACGCTA139550.018.4669623
ACGCTAC139750.018.37972624
CGCTACC145900.017.63587425
AAAACGC157950.016.48106821
GACCGCG52350.016.021795
AAACGCT167500.015.53025222
GAAAACG169000.015.32277320
CCGGCTT187450.015.2108222
GTCCGTT34750.014.9524521
AGACCGC56900.014.89868454
TCACGCT182800.014.7736278
TTCACGC184900.014.70325857
ATGACGG76950.014.49392716
ACCGCGT58050.014.4873436
GCCGGCT172400.014.4171771
Produced by FastQC (version 0.11.5)