FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004998979

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004998979
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44598217
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGGG98650.011.76239638
GTTGTCG23150.010.88684626
GGCATAG108100.010.566401535
AGCTTAG112400.010.40954145
GTTCTAT98050.09.7050581
TACGGTT31800.09.61614235
AACTCTA107550.09.43540945
TAATCGG50900.09.3375464
CGTGGCG87300.09.3024711
CATAGGG125550.09.16954337
ATCGGTT59000.09.1620416
GAGCTTA130800.09.08188344
GTCACTC162700.09.0533048
CGACCCG89100.08.973956
GGGAGGT182900.08.9535993
AATCGGT59050.08.6972335
GCATAGG134050.08.63875136
TGGCATA133800.08.63770934
GGAGGTC187500.08.6376534
GTAACGA49350.08.57752918
Produced by FastQC (version 0.11.5)