FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004998986

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004998986
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30114098
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA500800.16630084686581015No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG475760.15798580452251965No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG407750.1354016979024243No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACTC190900.015.4910468
GGTCACT192200.015.3739547
GGGAGGT215150.014.5249023
AGCTTAG167200.014.4273845
ACCCGGC57200.014.2783765
AGGGATC131100.014.1240152
ACTCTCC208800.014.079020511
AGGTCAC222900.013.6395296
GGAGGTC224200.013.6374084
CGTGTAA14050.013.61355445
TCACTCT218900.013.5917389
GGCATAG181300.013.50644235
GAGGTCA228900.013.416995
ATAGGGG187600.013.14842938
CACTCTC224700.013.00170210
ATAGCTC56900.012.89415239
TCAGACG78750.012.6565152
TGGGAGG212950.012.6255782
TCACCCG66900.012.6112313
TGCCCGT108650.012.55089544
Produced by FastQC (version 0.11.5)