FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004998987

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004998987
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30114098
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT962910.3197538906860169No Hit
CTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAG531900.17662823571869893No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG479710.15929748252795087No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCGC411250.1365639442363507No Hit
CCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAA301690.10018231328064352No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG25050.028.9202126
CGGTATA35000.026.29204820
AGACCGC196050.025.8324494
GACCGCG203200.024.9232755
CAGACCG207900.024.5234223
ACCGCGT208300.024.2914546
TCGACGA32350.023.0200927
TCAGACC227200.022.634962
CGACGAG31700.022.4275138
GTTCGAC34150.021.0161555
TTCGGCG103050.020.43547444
CCGCGTT248900.020.3471667
TCGGCGC104050.020.0660645
CGCGTTC262950.019.2428598
CTCAGAC301000.019.0460721
GTATAGA53050.018.44943622
CTACACG43200.017.9155845
GCGTTCT291850.017.4144549
AGTTCGA43050.017.1418464
ATGACGG51900.016.68979516
Produced by FastQC (version 0.11.5)