FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004998988

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004998988
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44303958
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTAGTT139300.015.27343344
AACGCTA70000.015.24011923
ACGCTAC72050.014.86714524
GTCCGTT34450.014.6255221
CCGTACG125800.014.2464
CGCTACC58100.013.81789225
CGTACGC130950.013.6857355
GGTTAGT155900.013.53203743
TTCGGCG130250.013.29565344
TCCGTAC137100.013.285013
AGACCGC37950.013.2724634
TCGGCGC131750.013.16152745
TACGCCA141550.012.8833637
GTCCGGT37200.012.879211
CTCGGTT73550.012.8445581
CGCCGCG132700.012.81418428
AAAACGC95100.012.77523121
CGCGCCC138750.012.76136921
CGCCCCG138650.012.69389623
GTACGCC143100.012.6651756
Produced by FastQC (version 0.11.5)