FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004998990

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004998990
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45185436
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAG803590.17784270135182495No Hit
CCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAA475440.10521974381302861No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG36850.027.7107566
CGGTATA62450.024.16725520
TCGACGA44500.023.5030867
AGACCGC89700.021.6910764
CGACGAG47250.021.469038
GACCGCG94600.020.6150975
CAGACCG97700.020.3064673
ACCGCGT102050.019.108926
TTCGGCG107450.019.0344344
TCGGCGC112050.018.04980745
GTATAGA90250.017.82013522
ATGACGG81550.017.21164516
AACGCTA113450.017.09060123
ACGCTAC114500.016.93415524
ACGAGGA84500.016.74323510
AACGACG27350.016.36656416
ATAGAGT99800.016.02510324
CGCTACC121000.015.9885125
CAGTTCG69950.015.75682453
CCGTACG133150.015.5250064
Produced by FastQC (version 0.11.5)