FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004999006

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004999006
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37426135
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT941240.2514927069012069No Hit
CTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAG920210.24587363883553565No Hit
CCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAA461160.12321870799643084No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC184050.025.3318944
GACCGCG189850.024.5205045
CAGACCG190700.024.519333
ACCGCGT198600.023.4959056
TCAGACC221250.021.5608732
CTCAGAC269200.019.0679511
CCGCGTT254850.018.2922787
ACGCTAC90150.018.09406324
AACGCTA90300.018.06400723
CGCTACC94300.017.29772625
CGCGTTC278450.016.766168
TTCGACG15200.016.134136
AAAACGC105700.015.68797121
GCGTTCT319350.014.7386419
AAACGCT113450.014.53694522
TTCGGCG49250.014.483744
GAAAACG120450.013.80508820
CGTACGC56150.013.5840565
CGGTATA22950.013.530004520
GTCGGTT36900.013.4778211
Produced by FastQC (version 0.11.5)