FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004999022

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004999022
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35125637
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAG1235840.3518341888006188No Hit
CCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAA703640.20032092229387896No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT656010.18676102585698304No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG462950.13179832155072377No Hit
CTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACG402280.11452603692283217No Hit
CCTGCTGCCACCTGCTCTTGTCCACGGTGAGCTTGCTGTAGAGGAAGAAGG389060.11076240410956817No Hit
CTCAACTTTCTTGTCCACCTTGGTGTTGCTGGGCTTGTGATTCACGTTGCA370580.10550128955668477No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC129750.024.5807134
CAGACCG140450.022.9802723
TTCGACG14700.022.4917476
GACCGCG150450.021.2734685
ACCGCGT152600.021.0031036
CGGTATA23400.020.57039820
TCAGACC163550.019.9827332
TTCGGCG78700.018.81382844
TCGGCGC83500.017.86565845
TCGACGA20300.017.505947
AACGCTA175150.016.83560423
ACGCTAC175700.016.77010224
CTCAGAC222200.016.7467141
CGCGTGT10900.016.3056741
CGCTACC180850.016.26854925
AAAACGC190150.015.61420621
GTCCGTT21900.015.4093861
CCGTACG97100.015.1258434
GTTTTCG197150.015.08782545
GAAAACG196950.015.07518720
Produced by FastQC (version 0.11.5)