FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004999025

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004999025
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27294275
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT3803741.393603603686121No Hit
CCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAA677500.24822055174574154No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA512600.18780495177102158No Hit
CGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAAT275530.10094790940591021No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAGACC612900.030.7693982
CAGACCG609750.030.7660223
AGACCGC611400.030.6278044
GACCGCG615800.030.3668825
CTCAGAC639450.030.1506941
ACCGCGT623200.030.0351776
CCGCGTT706000.026.5122537
CGCGTTC740250.025.2977318
GAACGAT724000.024.7677345
GCGTTCT764600.024.5097359
CGTTCTC806950.023.30984110
AGAACGA793250.022.63952444
AAGAACG817650.021.96918943
ATACGGA832500.021.66736233
GTTCTCT873850.021.6102411
AATACGG834700.021.5999432
TACGGAG834400.021.55609534
CAATACG845500.021.3354631
CACTCCC855250.021.24813824
CCTCTCA858250.021.10952219
Produced by FastQC (version 0.11.5)