FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004999034

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004999034
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43870662
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAG856240.19517371312974488No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG619970.1413176760359805No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG19300.019.6881776
CCGCGTT97500.016.8839361
AGACCGC49150.016.5142884
AACGCTA219250.016.33381723
ACGCTAC221150.016.15916624
GACCGCG50500.016.1173385
CGCTACC225050.015.83909225
CCCGTAT27350.015.7874181
GCCGGCT213000.015.710521
TCGACGA27600.015.478267
CCGGCTT251700.015.1590432
AAAACGC240200.014.94988621
CGCGTTC108350.014.8578952
CGTGGTT236400.014.77230141
GTCCGTT30750.014.7000281
CGGTATA35700.014.69171520
AAACGCT246700.014.63016822
CGGCAGT302850.014.52330440
ACCGCGT56750.014.3819216
GAAAACG252150.014.28386820
Produced by FastQC (version 0.11.5)