FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004999036

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004999036
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33860049
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT3719551.0985069749899063No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACG702120.2073594164024984No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA614130.1813730393597481No Hit
CCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAA367000.1083873209988562No Hit
CCACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAAC351170.10371219486421888No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC631550.027.9108984
CAGACCG641500.027.4818153
ACCGCGT652300.027.0641376
GACCGCG653450.026.9857275
TCAGACC659350.026.9086282
GAACGAT659150.026.63981245
CTCAGAC701600.025.9737321
CCGCGTT700650.025.202937
CGCGTTC715950.024.6549158
AGAACGA728700.024.1318744
GCGTTCT740800.023.8157259
CGTTCTC792800.022.36145210
AAGAACG802900.021.83873243
ATACGGA824200.021.30841433
AATACGG826300.021.28355232
TACGGAG827000.021.24288634
CAATACG828650.021.23887831
TTCGACG13050.021.1993146
CCTCTCA840250.021.09098619
ACGGAGA842750.020.81850635
Produced by FastQC (version 0.11.5)