FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004999037

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004999037
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33860049
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG1155420.3412340011675707No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA1001640.29581764633595187No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG572790.16916396074914125No Hit
CAGGGATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAG492320.1453984901203185No Hit
ATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGA449270.1326843915671829No Hit
CTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAAC445310.13151487170027426No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG136600.020.18092745
ATAGGGG153250.018.00371638
GGCATAG159450.017.27118735
GAGCTTA164150.016.78716944
CATAGGG171900.016.037537
GCATAGG181550.015.22233136
TGGCATA185550.015.10006234
AGGGATC176350.014.6319522
GGGAGGT240350.014.4626173
GTTGTCG21700.014.44155326
GGAGGTC251450.013.7548164
CAGGGAT204550.013.5701951
AGCGCTC55600.013.4489279
ATATAGC26100.013.416586
ACTCTCC257100.013.30639911
TGTCGTT25050.012.96194928
CATTTCT467250.012.6593661
GCATTCG38500.012.59023
CGCTCTC59400.012.36585211
TCGCGCT37750.012.30029845
Produced by FastQC (version 0.11.5)