FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004999044

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004999044
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38746889
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGGGAGAAGAACGAT1088200.28084835404463054No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC286750.025.2567964
GACCGCG292000.024.7245885
CAGACCG298600.024.383483
ACCGCGT302350.023.8484886
TCAGACC337050.021.8888782
ATACGGG244050.021.74985533
CCGCGTT333400.021.6204747
TACGGGG247550.021.5056134
CGCGTTC345300.020.8487728
GAACGAT349250.020.31029945
CTCAGAC405450.019.5932831
ACGGGGA275900.019.38486935
GCGTTCT379700.018.9952229
AGAACGA389200.018.17361344
CGGGGAG304200.017.5593136
AATACGG407450.017.17537332
AAGAACG418200.016.81670843
TTCGACG12400.016.692026
CAATACG425000.016.53521731
ACGCTAC39150.015.63418324
Produced by FastQC (version 0.11.5)