FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004999045

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004999045
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38746889
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA576440.14877065356137367No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG492880.1272050512235963No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG390910.10088809968717746No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG117550.017.38809845
ATAGGGG137400.015.05164938
GGCATAG138700.015.0017835
GAGCTTA145200.014.26299244
CATAGGG149950.013.87931737
GTCACTC198150.013.7415588
GGTCACT204450.013.174257
TGGCATA159700.013.15455934
GCATAGG160100.013.06789436
ACTCTCC214050.012.77426911
AGGTCAC229150.012.0386946
GGGAGGT250250.011.9270813
TAGGGGA232200.011.48534439
GTTGTCG18750.011.39587526
GAGGTCA252250.011.3152335
ACCCGGC70700.011.1754025
ATAGCTC66950.011.16028739
TCACTCT251800.011.1446119
CACTCTC251650.010.97251910
ACCGGTA26350.010.93498645
Produced by FastQC (version 0.11.5)