FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004999046

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004999046
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48497809
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1188770.24511828977676087No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG652360.13451329316753258No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC289250.022.191384
CAGACCG301000.021.6474513
GACCGCG320600.019.9640755
ACCGCGT329550.019.4082326
CTCAGAC384000.018.1074311
TCAGACC368300.017.8628182
CCGCGTT359100.017.7671267
ACGCTAC239700.017.67778224
TTCGACG20650.017.6492126
AACGCTA240600.017.61933723
CGCGTTC366600.017.3849268
CGCTACC246300.017.18605225
GCGTTCT372400.017.1621199
AAAACGC262100.016.31990421
GAACGAT393050.015.90023945
GAAAACG273950.015.65479520
AAACGCT277750.015.40038122
CGCGCTA1209.6758864E-515.0162061
CGGTATA40100.014.98175420
TCGACGA24200.014.96707257
Produced by FastQC (version 0.11.5)