FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004999051

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004999051
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33628028
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG458440.13632675695405036No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG118750.018.31849545
ATAGGGG127650.017.31998438
GGCATAG134500.016.3500135
CATAGGG142850.015.3982237
GAGCTTA146050.014.97465344
GCATAGG152200.014.43744836
TGGCATA155750.014.18632234
GTCACTC187700.014.1629538
ACCGGTA35200.013.70462645
GGTCACT193600.013.6952167
AGGTCAC201450.013.4160146
ACTCTCC205100.013.24124411
GGGAGGT213450.013.1836923
TAGGGGA203650.012.90350539
GGAGGTC216650.012.6698254
GAGGTCA221150.012.3823255
TACCGGT41050.011.87613644
TCACTCT232350.011.7043359
CACTCTC235050.011.34228110
TCTCCCC251350.010.77885913
Produced by FastQC (version 0.11.5)