FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004999058

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004999058
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37813373
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAG1371440.36268650247096446No Hit
CCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAA699690.1850377113937971No Hit
CTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACG383400.10139270040786894No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG23500.020.6664736
AGACCGC76050.019.6903924
CGGTATA35650.019.26002920
CCGCGTT185700.019.1712061
CAGACCG84500.017.9874383
GACCGCG86200.017.424055
TCGACGA29950.016.66627
CGCGTTC213450.016.611622
GTTCGAC30500.015.9970455
CGGCAGT425300.015.93046440
AGTTGCA439950.015.43585344
CGACGAG32150.015.2459628
ACCGCGT99000.015.2393976
TTCGGCG77950.014.94309844
GACGGCA451750.014.90278438
ACGGCAG455000.014.826439
TGACGGC458350.014.77174837
AACGCTA141000.014.58459223
ACGCTAC142550.014.48051224
GCGTTCT255500.014.4761913
Produced by FastQC (version 0.11.5)