FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004999059

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004999059
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37813373
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA718260.189948672391643No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG641000.16951674742160663No Hit
GTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGT466880.12346954607831467No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG434190.11482445641651698No Hit
CCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGG410840.10864939237237577No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG139800.014.43353345
ATAGGGG153450.013.43526238
GGCATAG156350.013.04121635
GTCACTC214600.012.8776128
GGTCACT218350.012.5017417
GGGAGGT238900.012.1113233
TGGCATA172000.011.97156734
AGGTCAC238750.011.8582786
GAGCTTA172850.011.75104344
ACTCTCC238150.011.69677611
GGAGGTC244400.011.6081254
CATAGGG179050.011.48967537
AGGGATC136900.011.3071262
TCACTCT255700.011.071939
GAGGTCA264400.010.9769335
CACTCTC254350.010.71369610
GCATAGG192550.010.70650536
TATCCTA78700.010.3075245
ATTTCTA337450.010.0411352
CAGGGAT184350.09.6008221
Produced by FastQC (version 0.11.5)