FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004999060

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004999060
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45298108
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCA3684680.8134291171719578No Hit
CAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAAC953850.21057170864619779No Hit
CTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA899690.19861535938763714No Hit
CAACTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGACTCC626350.13827288327362372No Hit
GTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA515530.11380828532617743No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA485640.10720977573721181No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG464940.10264004845412086No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGACT644850.029.9920442
CACAGAC682100.028.9440941
AGACTCA748800.025.7835854
CAGACTC789550.024.6085663
GACTCAG804350.024.1032685
ACTCAGA825300.023.4907916
AACCCAC927150.020.4080516
TCAGAGA951650.020.1319188
GAACCCA955950.019.78801215
AGAACCC958050.019.76843314
ACCCACC978150.019.67109917
AGAGAAC990600.019.22824712
CCGACAA985900.019.1035940
GAGAACC997350.019.0278513
CAGAGAG1018850.018.844349
TGCCGAC1014750.018.75067738
GCCGACA1012500.018.67129539
CTCAGAG1034150.018.6210887
CTGCCGA1022500.018.60919437
CCTGCCG1038700.018.32567836
Produced by FastQC (version 0.11.5)