FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004999061

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004999061
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45298108
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1238770.2734705829214765No Hit
GCGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAACATCCTCTCCAGGG648810.14323114775566342No Hit
GTCCAGGTGAGCCAGGCCATCACTAAAGGCACCGAGCACTTTCTTGCCATG469810.10371514854439394No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGCG278600.025.2997175
AGACCGC279700.025.2340074
ACCGCGT282050.024.9505636
CAGACCG301200.023.6503123
CCGCGTT309950.022.6971447
TCAGACC324500.022.2848992
CGCGTTC328550.021.363758
GCGTTCT367950.019.1674149
CTCAGAC470150.017.2429051
AACGCTA167100.017.10159123
ACGCTAC167350.016.983524
TTCGACG15000.016.7983466
GAACGAT417700.016.6852345
CGCTACC174400.016.37453525
TTCGGCG47350.016.24898344
GCCGGTT112500.015.9977641
CGTTCTC450250.015.95359410
CTACACG63400.015.82562445
AATACGG436850.015.70010132
ATACGGA437800.015.696506533
Produced by FastQC (version 0.11.5)