FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004999076

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004999076
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36945419
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG19650.09.84641426
ATAGGGG56600.09.02338138
CGTGGCG72450.08.2318471
CATAGGG62800.07.98914637
GGCATAG62100.07.97051235
CGACCCG76700.07.89252766
AGCTTAG64800.07.812018445
GCATAGG65600.07.545103536
ATATAGC36150.07.53245076
ACCGGTA18900.07.49953845
ATAACGC30050.07.487190239
TTAACGG34400.07.45713663
TGTCGTT28050.07.298293628
CACCGAA103050.07.29212745
TGCCCGT100850.07.16117544
GCGACCC84600.07.155495
TTTAACG45950.07.14973742
TACCGGT20150.07.145951744
GTGGCGA82150.07.0943882
TAACGCC32350.06.954852640
Produced by FastQC (version 0.11.5)