FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004999084

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004999084
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40168427
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG496790.1236767374535229No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG131700.018.4649745
ATAGGGG141600.016.8963938
GTTGTCG24350.016.08086626
GGCATAG149000.016.01190835
CATAGGG159850.014.92545337
GAGCTTA162900.014.90022644
GTCACTC218100.014.6587158
GGTCACT228750.014.0555457
GCATAGG172200.013.86810936
TGGCATA176600.013.61095334
ACTCTCC236100.013.50300411
GGGAGGT256400.013.2647913
AGGTCAC250950.013.1799946
ATATAGC34600.012.6893426
GGAGGTC260700.012.6651244
ACCGGTA30150.012.609797545
TAGGGGA252900.012.60833239
GAGGTCA270400.012.2854185
TGTCGTT32800.012.06255328
TCACTCT273000.011.9005029
Produced by FastQC (version 0.11.5)