FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004999085

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004999085
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40168427
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT665120.16558278470799964No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC141750.024.8393694
GACCGCG145100.024.2813575
ACCGCGT152000.023.1791366
CAGACCG157250.022.7484593
CCGCGTT164650.021.3846117
TTCGACG17900.021.1022196
CGCGTTC172200.020.4339858
TCAGACC195650.018.8122712
AACGCTA53550.017.96464323
ACGCTAC55000.017.54505524
GCGTTCT202100.017.4994149
CGGTATA30800.017.3981920
TCGACGA21950.017.3110567
GAACGAT205700.017.12011545
TTCGGCG52400.016.65131644
CTCAGAC247750.016.290871
CGCTACC61100.015.86722325
TACGGAG224250.015.59945334
ATACGGA225600.015.57590933
AATACGG225300.015.54672232
Produced by FastQC (version 0.11.5)