FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004999089

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004999089
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50589265
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAG1606530.31756341982829756No Hit
CCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAA761120.15045089111296636No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG30250.022.8266436
AGACCGC96250.020.3311614
CGGTATA49150.020.31907720
GACCGCG103200.019.0709365
CCGCGTT208900.018.7663061
TCGACGA37250.018.5976947
CGACGAG38200.017.9587388
CAGACCG111000.017.913193
CGGCAGT469450.017.84509840
ACCGCGT114050.017.1574616
TGACGGC509600.016.44268637
TTCGGCG109800.016.23274244
GACGGCA515200.016.21152738
CGCGTTC242650.016.0460032
ACGGCAG524300.016.02238339
AGTTGCA527800.015.87849544
CTGACGG535700.015.63331336
CAGTTGC541050.015.58983643
TCGGCGC117600.015.23065445
GCAGTTG566550.014.86035842
Produced by FastQC (version 0.11.5)