FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004999097

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004999097
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30281438
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG554930.1832574793839051No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA486890.1607882690379499No Hit
CTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGC315820.10429491492444977No Hit
CTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAAC304320.1004972088842016No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGGG90250.015.31013138
AGCTTAG92800.014.91878745
GGCATAG95950.014.48808635
CATAGGG97350.014.39895237
AGGGATC122800.014.3335962
ATAGCTC48150.013.55473539
GCATAGG105750.013.25349636
ACCCGGC53300.013.17721755
GAGCTTA109900.012.78192144
GGTCACT137000.012.762027
GTCACTC138000.012.6866238
TGGCATA113850.012.42614234
CAGGGAT159600.011.8744181
GGGATCA150750.011.8414543
ACGGACA59700.011.71854518
ACTCTCC153250.011.70386311
TAGCTCT56800.011.49177540
TCACCCG62950.011.4073333
TACCGGT16800.011.25587544
TGCCCGT104000.011.23422944
Produced by FastQC (version 0.11.5)