FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004999099

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004999099
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35147045
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA785620.22352376992148273No Hit
CCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGG680380.19358099663855097No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG644330.18332408883876297No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG524500.14923018421605572No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATAGC57600.020.3017376
GGAGGTC289900.017.8195824
GGGAGGT297700.017.6852633
CAATATA69600.017.6181834
GTTGTCG61350.017.0918326
GAGGTCA306100.016.913645
TATAGCT69650.016.7575177
AATATAG80000.016.3406725
GCTAAGT69200.016.31602314
TACAATA77200.016.0292662
ACAATAT87050.014.9394133
TCGTTCC74750.014.78267830
TGGGAGG249700.014.7412082
AGGTCAC352650.014.7369776
GTCGTTC73000.014.70467529
TGTCGTT74100.014.54602828
TTGTCGT74700.014.36865827
ACCCGGG76150.014.034342
GGTCACT366100.013.9379297
GTCACTC370400.013.7880898
Produced by FastQC (version 0.11.5)