FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004999105

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004999105
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43968714
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCA6253981.4223704609600363No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG2193950.4989797973167921No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA1711890.38934274948318937No Hit
CTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA1288170.29297422708337567No Hit
CAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAAC1244710.28308992616886636No Hit
CCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACC1148280.2611584227821628No Hit
ATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGA887100.2017570948288367No Hit
CAGGGATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAG785950.17875210086881324No Hit
GATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCA703590.16002060010215444No Hit
CAACTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCATCTGACTCC696370.15837852342008457No Hit
GGGATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAG686050.15603139996316473No Hit
CTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAAC678220.15425058827055982No Hit
CAACTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGACTCC623570.14182129593328566No Hit
CTTCAAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCACCTCCC576880.13120238176627136No Hit
GGATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGC571140.12989690806058143No Hit
CTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCATCTGACTCCTGA549300.12492973981454175No Hit
TCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAA525680.11955773825907212No Hit
ACTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCATCTGACTCCTG503500.11451324230224244No Hit
AGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGCC500080.1137354165054725No Hit
CTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGACTCCTGA494020.1123571637778626No Hit
TTCAGGGATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGT489900.11142013387064266No Hit
TTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAA478070.10872958440403785No Hit
GTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGT461570.10497691608628808No Hit
GTCAACTTCAAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCAC449290.10218402112010827No Hit
CTTCTCCCTCGAGTTGGGACCCGGGAAGAACCATGAAGTGGCTGCTGCTGC440990.10029631523905838No Hit
ACTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGACTCCTG440570.1002007927727884No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGACT985450.031.1696722
CACAGAC989850.031.083011
AGACTCA1109500.027.6788734
GACTCAG1144250.026.8887945
CAGACTC1143450.026.8883273
ACTCAGA1304800.024.1581956
TCAGAGA1334600.023.0045328
CTCAGAG1400100.022.0452167
AACCCAC1376000.022.0097716
GAACCCA1403850.021.60511615
CAGAGAG1417250.021.5459339
AGAACCC1409050.021.52182814
ACCCACC1416350.021.47642117
AGAGAAC1434950.021.17961312
AGAGAGA1444450.021.12142610
GAGAACC1440200.021.091813
CCGACAA1469850.020.43378340
GAGAGAA1494000.020.40258411
GCCGACA1472350.020.35227239
TGCCGAC1480600.020.26849738
Produced by FastQC (version 0.11.5)