FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004999111

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004999111
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40413346
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA661820.16376273323173982No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG647840.1603034799444718No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG420210.10397802745657338No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG152550.014.91365445
GTTGTCG27300.014.84373826
GGCATAG161900.013.95244435
ATAGGGG162550.013.89837638
GGGAGGT238350.012.8342183
GAGCTTA179050.012.80769544
TGTCGTT33650.012.57976428
TGGCATA180850.012.56427934
GGAGGTC243050.012.4211624
AGGGATC143500.012.254782
CATAGGG185800.012.17019937
ATATAGC41100.012.1305796
ACTCTCC257100.011.57562911
GCATAGG196050.011.56802836
GGTCACT253750.011.4987857
GTCACTC261700.011.3309798
GTCGTTC38700.010.88042729
GCTAAGT38500.010.86375314
TATAGCT44400.010.725117
CAGGGAT191500.010.2971251
Produced by FastQC (version 0.11.5)