FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004999112

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004999112
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32767348
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAG1708530.5214123523209752No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG961140.29332248676334743No Hit
CCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAA789190.24084646703785734No Hit
CTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACG560660.17110325803601803No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT494820.15101008479538838No Hit
CCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAGCC385520.11765370819756302No Hit
CCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGG361270.11025304824790826No Hit
CCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCA348360.10631315051800957No Hit
CCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAGCCA328730.10032243073195915No Hit
CTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAG328120.1001362698012668No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC117700.023.4741024
TTCGACG17600.023.0099856
CAGACCG124500.022.6094263
TTCGGCG84650.021.10231644
TCGGCGC89900.019.8198745
CGGTATA28500.019.73680520
GACCGCG149400.018.4776125
TCAGACC156350.018.3922372
CCGTACG104850.017.4028154
CGTACGC105550.017.3081115
CCCGCGT14500.017.2433151
TCGACGA24500.016.7126987
CTACACG95050.016.6867745
TACGCCA111900.016.3255257
ACCGCGT169350.016.2877756
TCCGTAC114800.016.2485353
AGTTGCA551550.016.19355644
CGACGAG25300.016.0952348
CGGCAGT553050.015.94299940
CTCAGAC213350.015.8578031
Produced by FastQC (version 0.11.5)