FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004999132

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004999132
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38382177
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAG557880.145348712242143No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC86150.022.094974
GACCGCG89700.021.0939065
CAGACCG93650.020.8322453
ACCGCGT94100.019.9405546
TTCGACG19250.019.6353826
CGGTATA35400.016.01681520
CCCGGTT45250.015.7298311
TCAGACC131750.015.4398112
TCGACGA25800.015.434637
AACGCTA131700.014.7955223
ACGCTAC134000.014.57560624
CGGCAGT207150.014.2495740
GACGGCA211250.013.93075638
CGCTACC140600.013.87556325
ACGGCAG213450.013.86063139
CCGCGTT139750.013.7000671
CCCGAAT60100.013.6796181
CGACGAG26550.013.5580428
AAAACGC145400.013.49417321
CGTTGTG187500.013.47465918
Produced by FastQC (version 0.11.5)