FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004999138

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004999138
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16688787
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAG474290.28419680831207206No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG291460.17464420871331152No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT262390.1572253274009669No Hit
CCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAA210750.12628239547907225No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG14700.026.1717936
AGACCGC60450.023.7462334
CAGACCG63300.023.1053
GACCGCG62950.022.8023515
CGGTATA24700.022.04510320
TCGACGA17950.021.6824847
ACCGCGT66950.021.4063956
GTTCGAC19200.020.6236785
CGACGAG19150.019.7361018
TCAGACC78000.019.1831872
AACGACG12050.018.109316
CAGTTCG25800.016.7447193
ATGACGG32100.016.46947716
AGTTCGA24550.016.3131894
CTACACG35300.015.99789345
TTCGGCG48750.015.9224444
TCGGCGC49650.015.72439845
CTCAGAC105600.015.5281431
CCGCGTT92700.015.4592017
ACGCTAC119550.015.17200624
Produced by FastQC (version 0.11.5)