FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004999143

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004999143
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11983274
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG506490.4226641233439209No Hit
CTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTTT319590.26669673079327066No Hit
CTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAG308570.257500579557807No Hit
CCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAA188670.15744445132440432No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCGC158160.13198396364799805No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT154800.1291800554673122No Hit
CCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGG147860.12338864987982416No Hit
GGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTTTCT132840.11085451271497254No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG7450.025.6697656
CGGTATA11600.022.49992220
AGACCGC41500.020.980894
GCCGGCT143750.020.9171661
CCGGCTT169750.020.3099042
GTTCGAC9900.019.5444055
TCACGCT185000.019.2638348
CAGACCG46350.018.9799613
CGGCTTC193150.018.7775363
TTCACGC193350.018.5366337
AACGCTA187850.018.4580223
ACGCTAC188050.018.42642424
CGCTACC190600.018.13290625
CGTGGTT188100.018.1093841
AAAACGC193350.018.00256321
GTTTTCG167350.017.81365645
AAACGCT195450.017.78633722
GTTCGAA22600.017.72043645
GAAAACG197000.017.69156320
CACGCTC202900.017.653079
Produced by FastQC (version 0.11.5)