FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004999147

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004999147
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39721077
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG1118250.2815255991170632No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA1035390.2606651375540497No Hit
CTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAAC533570.13432918749912043No Hit
CAGGGATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAG520530.13104629564802586No Hit
ATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGA495820.1248254169946097No Hit
GTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGT451130.1135744632503293No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGGC72800.017.024285
AGGGATC205850.015.1917372
TCACCCG91550.014.07926753
ATAGCTC84000.013.97129139
AGCTTAG117350.013.78199645
ACGGACA89800.013.48691918
GGCATAG118450.013.47484435
CAGGGAT247900.013.3529561
ATAGGGG123650.013.18157638
CACCCGG97450.013.1344094
CGGACAC92900.012.87050713
CATAGGG127850.012.64379137
CGGACAG96500.012.56353719
CCGGACA98200.012.51977212
TAGCTCT94250.012.47675840
GGGATCA257600.012.2818463
GTCACTC179800.011.8002588
GCATAGG138350.011.61824236
TGATAGC104350.011.61302737
CACGGAC103750.011.58904317
Produced by FastQC (version 0.11.5)