FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004999150

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004999150
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40983208
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG24600.020.929476
TCGACGA29050.018.2651257
CCCGTAT26600.018.0041791
CGGTATA42500.017.27125420
CGACGAG29400.016.1358538
AACGCTA143050.016.09684223
ACGCTAC145050.015.91771324
CGCTACC150700.015.24612125
GTTCGAC35050.014.8176635
CCGCGTT43500.014.1623941
TCACGCT170000.014.0321198
TCGTTAC16300.013.93390144
GCACTAC45800.013.88296125
AAAACGC168250.013.80031721
GTCCGTT32750.013.662091
CCCGCGT12050.013.6210691
GTCCGAT21700.013.5733461
CGTGGTT171050.013.38362441
CCGCTAT25850.013.3077981
GAAAACG174050.013.272984520
Produced by FastQC (version 0.11.5)