FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004999151

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004999151
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40983208
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG91100.011.977303545
ATAGGGG95300.011.53814138
TAGCACG20850.010.67385529
GGCATAG97200.010.6414935
TCAGACG65150.010.6115112
CGTGGCG123700.09.9599691
GGCGACC126800.09.7165044
CATAGGG112800.09.66829537
CGGCTAA78800.09.64240431
GAGCTTA117800.09.49119344
CGACCCG139750.09.3123036
TGGCGAC134500.09.2610833
GCATAGG117400.09.1554536
GTCACTC146750.09.1440518
GTGGCGA134250.09.1083322
GGGAGGT172750.09.0620353
AGACTCA136750.09.0424674
GCGACCC137600.09.0190285
TAATCGG39750.08.9528624
GGAGGTC158550.08.8788294