FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004999154

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004999154
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50632248
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAG958030.18921340407402018No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGCG109050.022.6272355
AGACCGC111150.022.3211524
ACCGCGT112150.022.0006356
TTCGGCG95200.021.48406444
TTCGACG25350.020.5843836
TCGGCGC103800.019.8966945
CAGACCG127600.019.6905463
CCGTACG117850.017.425764
CGTACGC119350.017.1879065
CGGTATA41900.017.12461320
AACGCTA125900.016.77614423
CCGCGTT147450.016.7642677
ACGCTAC127500.016.65415824
TACGCCA128350.016.1221757
TCGACGA33300.015.940417
CGCTACC133800.015.85410525
GTACGCC129950.015.7850666
CGCGTTC158400.015.6056218
TCAGACC168250.015.6017632
TCCGTAC134750.015.3906663
Produced by FastQC (version 0.11.5)