FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004999168

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004999168
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43649890
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAG1005800.2304244065678058No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT681230.1560668308671568No Hit
CCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAA657840.15070828357184862No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC154100.023.465234
GACCGCG161450.022.2832325
CAGACCG170300.021.5105653
ACCGCGT170600.021.1012766
TTCGACG19050.020.9048026
TCAGACC206550.018.1602762
CGGTATA36700.015.94062620
CTCAGAC259900.015.8545051
CGGCAGT325700.015.79708240
TCGACGA26800.015.5300327
AACGCTA92600.015.48054623
ACGCTAC92350.015.44943724
GACGGCA334650.015.21365738
AGTTGCA344700.014.97182144
CGCTACC96750.014.93294125
TTCGGCG50550.014.8199144
TGACGGC346800.014.7651437
ACGGCAG351450.014.65895139
GTCCGTT34950.014.2459161
CCGCGTT253750.014.2211187
Produced by FastQC (version 0.11.5)